Journal: Cell Reports Medicine
Article Title: Intestinal Lachnospiraceae bacterium -derived propionate inhibits the progression of clear cell renal cell carcinoma
doi: 10.1016/j.xcrm.2025.102410
Figure Lengend Snippet: L. bacterium protects against ccRCC tumorigenesis through its supernatant (A) Experimental design: After gut microbiota depletion with ATB, RENCA-bearing mice were gavaged with L. bacterium or P. copri 5 times/week ( n = 6/group). (B and C) Tumor growth kinetics (B) and tumor weights (C) on day 24 in mice receiving L. bacterium or P. copri ( n = 6/group). (D) 16S rRNA sequencing of fecal samples from control and L. bacterium -treated mice on days 12 and 24. (E) Experimental design: RENCA-bearing mice pretreated with ATB were gavaged with L. bacterium with or without amoxicillin in drinking water ( n = 9/group). (F and G) Tumor growth curves (F) and tumor weights (G) on day 24 in the indicated groups ( n = 9/group). (H–J) Effects of L. bacterium SN on 786-O and Caki-1 cells: colony formation (H), cell viability via CCK8 assay (I), and wound healing assay (J) with 100× and 10× dilutions of SN. Scale bars: 200 μm. (K) Experimental design: ATB-pretreated RENCA-bearing mice were gavaged with L. bacterium , heat-inactivated L. bacterium , or SN ( n = 9/group). (L and M) Tumor weights in BALB/c (L) and BALB/c-nude mice (M) on day 24 ( n = 9–10/group). Data are shown as mean ± SEM. Statistical significance was determined by two-way ANOVA (B, F, and I), unpaired two-tailed t test (C, G, L, and M), and paired two-tailed t test (J). ∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗ p < 0.001. See also and .
Article Snippet: The human cell lines 786-O, Caki-1, and 293T, as well as the murine renal carcinoma cell line RENCA, were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA).
Techniques: Sequencing, Control, CCK-8 Assay, Wound Healing Assay, Two Tailed Test
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